Mudanças no estilo de vida de isolados selvagens e veterinários de Pseudomonas aeruginosa expostos a três antibióticos de uso veterinário
DOI:
https://doi.org/10.54580/R0701.08Resumo
Este estudo teve como objetivo avaliar a atividade planctônica e séssil de isolados silvestres e veterinários de Pseudomonas aeruginosa expostos por 4 h à formulações veterinárias compostas de florfenicol (FLO) e sulfametoxazol+trimetoprima (SXT), sob diferentes níveis de Demanda Química de Oxigênio (DQO). Foram realizados testes adicionais para produção de piocianina, antibiograma e hidrofobicidade da parede celular. Todos os isolados produziram até 3,26 µg/L de piocianina e apresentaram parede celular fortemente hidrofóbica. Tanto o DQO de 20.000 mg/L como a presença de FLO favoreceram a inibição da adesão celular em até ≈45%, e nenhum efeito antibiofilme foi encontrado. FLO/SXT nas concentrações 0,30/0,24 mg/mL exibiram as maiores atividades. Foi observado um aumento de até ≈16% na presença de FLO e percentuais menores na presença de SXT. Houve um aumento significativo nas células planctônicas em até ≈32%. Dois fatores podem ter sido responsáveis por isso: descolamento ativo no biofilme e duplicação de células tolerantes, pois as velocidades de natação e corrida foram reduzidas, mas as células permaneceram viáveis (SXT > FLO). Os resultados do antibiograma indicaram que os isolados veterinários de P. aeruginosa foram mais sensíveis aos antibióticos do que os isolados selvagens, porém, no teste in vitro de formação de biofilme os isolados selvagens foram mais suscetíveis a alterações do que os isolados veterinários. No meio com maior teor de matéria orgânica, entretanto, a passagem do estado séssil para o planctônico parece ter servido como estratégia de sobrevivência para P. aeruginosa em concentrações subinibitórias de FLO e SXT.
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